It is well known that miRNAs are involved in many essential cellular processes, such as cell proliferation, differentiation, apoptosis, stress response and tumorigenesis etc. MiRNAs function in RNA silencing and post-transcriptional regulation of gene expression. Mature miRNAs assemble into RNA-induced silencing complex (RISC), guide the complex to target messenger RNAs (mRNAs), and regulate their expression either by cleavage of target mRNA or by translation inhibition.
Creative Biogene is developing stable cell lines overexpressing human / mouse/ rat miRNA precursors covered in the current miRbase. Our miRNA overexpression stable cell lines are constructed by lentivirus transduction or non-viral plasmid transfection of vectors optimized by our scientists to enable expression and maturation of miRNAs inside cells. These cell lines are valuable tools for studying miRNA gain-of-functions and identifying the potential target genes.